CV - igtrcn

Curriculum Vitae
PERSONAL INFORMATION
Valeria Petrella
Via F. Caiazzo, 14, 80038 Pomigliano d'Arco (Italy)
+39 3495876105
valeria.petrella@unina.it
Sex Female | Date of birth 05 July 1984 | Nationality Italian
JOB APPLIED FOR
Biologist
WORK EXPERIENCE
September 2009–September
2010
Master student
Universidad de Granada, Granade (Spain)
During my experience as master student in the Prof. Rafael Jimenz Medina genetic lab, I worked on
the mole Talpa occidentalis reproductive system. The aims of my work have been cloning several
genes envolved in the reproductive mechanisms and to study their expression by
immunohistochemical experiments.
April 2011–Present
Ph.D. Student
Università degli Studi di Napoli "Federico II", Naples (Italy)
During my Ph.D. in the insect molecular genetic lab, I'm mainly working on the diptera Ceratitis
capitata, expecially about sex determination.
The first part of my Ph.D. experimental work has been focused on the study of a Ceratitis capitata
transgenic strain . The main goal has been the identification by (inverse-PCR) and characterization of
the sequences of the genomic regions flanking the transgene insertion point and to investigate the
presence of an enhancer that could be responsible of the transgene expression. I did mating
competition tests and molecular analysis of several genes envolved in sex determination. I've a good
experience on embryos microinjections and transgenic lines productions, as well on RNAi.
During my two months visit to Oxitec ltd. (Oxford) I've adquired practice working on other insects, like
mosquitoes, and new revers genetic strategy, like TALENs.
During my visit to Prof. L.Rabinow at the Centre de Neurosciences de Paris Sud (Universitè Paris Sud
XI-Paris) I've worked on the CcDOA gene: starting from the DmDOA gene, I've obtained the gene
structure and sequence of the locus DOA in Ceratitis capitata and studied studied some of the DOA
isoform in several developmental stages.
In order to develop new sexing strategies, I've identify in Ceratitis several Drosophila melanogaster
homologues genes and I've analyzed their sex biased expression levels by qPCR.
Paper and Communication
The orthologue of the fruitfly sex behaviour gene fruitless in the mosquito Aedes aegypti: evolution of
genomic organisation and alternative splicing.Salvemini M, D'Amato R, Petrella V, Aceto S, Nimmo D,
Neira M, Alphey L, Polito LC, Saccone G.PLoS One. 2013;8(2):e48554.
Salvemini, M., D'Amato, R., Petrella, V., Nagaraju, J., Saccone, G. and Polito, L.C. Sottrazioni ai tempi
della crisi: approcci molecolari ed in silico per l'isolamento del segnale primario della determinazione
del sesso di Ceratitis capitata. XVI° Congresso della Drosophila Italiana - Napoli, 1-3 Ottobre 2012.
Salvemini, M., D'Amato, R., Petrella, V., Aceto, S., Neira, M., Nimmo, D., Alphey, L., Polito, L.C. e
Saccone, G. La determinazione del sesso nella zanzara Aedes aegypti: i geni alla base della cascata,
dsx e fru. XVI° Congresso della Drosophila Italiana - Napoli, 1-3 Ottobre 2012.
Saccone, G., Salvemini, M., Petrella, V., D'Amato, R., and Polito, L., C. A transgene-based maternallydriven RNAi system produces male-only progeny in the Mediterranean fruit fly, Ceratitis capitata. XXIV
International Congress of Entomology - Daegu, Korea, 19-25 Agosto 2012.
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Curriculum Vitae
Valeria Petrella
Saccone, G., Salvemini, M., D'Amato, R., Tomar, A., Petrella, V., Ciolfi, S., Dallai, R., Marchini, Franz,
G., Nagaraju, J., and Polito, L. C. Role of the Y chromosome in the Ceratitis capitata sex
determination and reproduction. XXIV International Congress of Entomology - Daegu, Korea, 19-25
Agosto 2012.
Salvemini, M., Sanges, R., D'Amato, R., Petrella, V., Sanfelice, D., Nagaraju, J., Saccone, G. and
Polito, L. C. Early embryonic sexed Ceratitis capitata transcriptomes: in silico approaches to identify
male biased/male determining genes. “Next Generation Sequencing - Application cases and
bioinformatics development” German-Italian Dialogue – Napoli, 17-19 Luglio 2012.
Saccone G., Salvemini M., D'Amato R. , Petrella V., Bopp, D., Polito C. Sex determination in Ceratitis
relies on a conserved binary genetic ON/OFF switch, splicing-based and epigenetic. EuroEvoDevo
2012 – Lisbona, Portogallo, 10-13 Luglio 2012.
Salvemini, M., D'amato, R., Petrella, V., Saccone, G. and Polito, L. Sex determination in Aedes
aegypti. Workshop “Nothiger Meeting” del Gruppo Europeo “Sex Determination in insects”.
Amsterdam - Olanda, 5-8 Ottobre 2011.
Salvemini, M., Nagaraju, J., D’Amato, R., Petrella, V., Pane, A., Polito, L. C., and Saccone, G. Toward
a mRNAseq analysis to explore the transcriptome landscapes of Ceratitis capitata sexed early
embryos. Workshop “Nothiger Meeting” del Gruppo Europeo “Sex Determination in insects”.
Amsterdam - Olanda, 5-8 Ottobre 2011.
D'Amato, R., Salvemini, M., Petrella, V., Pane, A., Saccone, G. and Polito, L.C. Early sex
determination steps in the mediterranean fruitfly Ceratitis capitata and its manipulation for production
of a male-only progeny. Joint Meeting AGI-SIBV- SIGA, Cittadella di Assisi (PG), 19-22 Settembre
2011.
Salvemini, M., Sanges, R., Nagaraju, J., D’Amato, R., Petrella, V., Pane, A., Polito, L.C. and Saccone,
G. Towards the analysis of transcriptome from sexed Ceratitis capitata embryos: how to produce XX
male embryos by transgene-mediated RNAi and identify male biased/male determining genes. 5th
Annual Arthropod Genomics Symposium: Exploring Diversity, Relating Similarity. Kansas City
(Missouri) USA - 9-11 Giugno 2011.
Saccone, G., Salvemini, M., D'Amato, R., Leo, S., Petrella, V. and Polito, L. C. Climbing up the
conserved sex determination cascade in tephritidae and mosquitoes. Second FAO/IAEA Research
Coordination meeting, Development and Evaluation of Improved strains of insects pests for SIT,
Nanjing, China, 9-13 Maggio 2011.
courses and meeting
Blast2Go and Babelomics: suitable tools for functional annotation and data mining.Portici (NA),
Facoltà di Agraria 12-16 Novembre 2012
Workshop “Nothiger Meeting” European Group “Sex Determination in insects”. Amsterdam Netherlands, October 5-8 2011.
Workshop “Nothinger Meeting” European Group “Sex Determination in insects”. Gif-sur-Yvette France June 12-14, 2013
EDUCATION AND TRAINING
October 2003–July 2007
bachelor degree in general and applied biology
University Federico II, Naples (Italy)
Immonohistochemistry
October 2007–February 2010
Mester Degree in Biology
University Federico II, Naples (Italy)
molecular biology, immunohistochemistry, genetic, bioinformatic.
April 2010–March 2011
master of advanced studies in human biology of reproduction
Seconda Università degli Studi di Napoli, Napoli (Italy)
cytogenetic, molecular biology.
April 2011–Present
PhD in biotechnologies
University Federico II, Naples (Italy)
molecular biology (RT-PCR, qPCR, inverse-PCR), reverse genetic (RNAi) and insect transformation,
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Curriculum Vitae
Valeria Petrella
bioinformatic.
PERSONAL SKILLS
Mother tongue(s)
Italian
Other language(s)
UNDERSTANDING
SPEAKING
WRITING
Listening
Reading
Spoken interaction
Spoken production
English
B1
C1
B2
B1
B2
Spanish
C1
C1
C1
C1
B1
Levels: A1/A2: Basic user - B1/B2: Independent user - C1/C2: Proficient user
Common European Framework of Reference for Languages
Communication skills
Organisational / managerial skills
My working experiences in Italy and abroad have allowed me to gain very good skills in adapting to
different opinions, in taking responsabilities and in resolving conflicts.
I'm very good at planning, coordinating resources and meeting deadlines.
I am used to be responsible not only for my own activities but also for the collective actions of the
entire working group.
Job-related skills
mentoring skills: my experience as PhD student has allew me to gain very good skills in teaching, in
supervising undergraduate and graduate students in their lab actvities and in their education.
Computer skills
good knowledge of Windows, Linux and office package. Furthermore I have good knowledge of the
bioinformatic tools for biological and statistical analysis
ANNEXES
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