Human Full-Length cDNA Clones

Human Full-Length cDNA Clones
20,000⒳㘃એ਄ߩࡅ࠻ቢో㐳cDNA⊒ᄁ㐿ᆎ㧍
⃻࿷䇮OriGene␠䈲20,000⒳㘃એ਄䈱䊍䊃ቢో㐳㩷cDNA 䉕଻᦭䈚䈩䈇䉁䈜䇯䈖䉏䉌20,000⒳㘃䈱䊍䊃ቢో㐳㩷
cDNA 䈱⚂90䋦䈏㩷NCBI RefSeq 䊂䊷䉺䊔䊷䉴਄䈱 NM sequnce ෸䈶㩷XN sequence 䈮⊓㍳䈘䉏䈩䈇䉎䊍䊃ቢ
ో㐳ㆮવሶ㈩೉䈫⋧หᕈ䉕␜䈚䉁䈜䇯ฦ䇱䈱䉪䊨䊷䊮䈲䇮ォ౮㐿ᆎㇱ૏䉕฽䉃ቢో㐳㩷cDNA 䈪䈅䉎੐䉕5㫪ᧃ┵
䈱㈩೉⸃ᨆ䈫㩷NCBI 䈮⊓㍳䈘䉏䈩䈇䉎ฦㆮવሶ㈩೉䈫䈱Ყセ䈎䉌᣿䉌䈎䈮䈘䉏䈩䈇䉁䈜䇯
㩷ォ౮‛䈏৻䈧એ਄䈱ផቯ㩷ORF 䉕᦭䈜䉎႐ว䉇㩷ORF 䈏䇮5㫪ᧃ┵䈎䉌㔌䉏䈢ਅᵹ䈮૏⟎䈜䉎႐ว䈮䈲䇮㩷NCBI
䈪⸥タ䈘䉏䈩䈇䉎㈩೉ᖱႎ䈱5㫪ᧃ┵䈱ᭂ㒢ㄥ㈩೉⸃ᨆ䉕ⴕ䈇䇮䈎䈧3㫪ᧃ┵䈱㈩೉⸃ᨆ䈎䉌䉅ㆮવሶ䈱⋧หᕈ
䉕⏕⹺䈚䈩䈇䉁䈜䇯෶䇮NCBI 䊂䊷䉺䊔䊷䉴䈮䈍䈇䈩䇮3㫪UTR䈱䊃䊤䊮䉬䊷䊃䉇ᰳᅤ䈏↢䈛䈩䈇䉎႐ว䇮ផቯ䈘䉏䉎㩷
ORF 䉋䉍ਅᵹ䈱㈩೉䈮૏⟎䈜䉎䉭䊉䊛㈩೉䈎䉌䈱⏕⹺䇮⚳⚿䉮䊄䊮ㄭற䈱਄ᵹ㈩೉䈮⋧⵬⊛䈭䊒䊤䉟䊙䊷䉕૶
↪䈚ផቯ䈘䉏䉎㩷ORF 䈱3㫪ᧃ┵㈩೉䈱⏕⹺䉕ⴕ䈦䈩䈇䉁䈜䇯OriGene␠䈲䇮䈖䉏䉌෩㊀䈭㈩೉⸃ᨆ䈱ⴕ䉒䉏䈢䊍
䊃ቢో㐳㩷cDNA 䉕ේᩭ䇮⌀ᩭਔ↢‛䈪⊒⃻น⢻䈭䊔䉪䉺䊷䈮ዉ౉䈚䈗ឭଏ⥌䈚䉁䈜䇯
․㩷㩷㐳
⵾ຠ⺑᣿
䃨 ⼾ን䈭ຠឥ䈋(20,000⒳㘃એ਄䈱䊍䊃ቢో㐳cDNA)
䃨 ⌀ᩭ䇮ේᩭ䈱ਔ䉲䉴䊁䊛䈪⊒⃻น⢻
䃨 䉝䊒䊥䉬䊷䉲䊢䊮䈏⼾ን
㩷㩷㩷䋨䊃䊤䊮䉴䉳䉢䊆䉾䉪䊙䉡䉴䇮䉶䊦䊐䊥䊷䈪䈱⊒⃻䇮⚵䉂឵䈋䉺䊮䊌䉪⾰⊒⃻䉅น⢻䋩
䊔䉪䉺䊷㩷pCMV6-XL4䇮pCMV6-XL5෸䈶pMV6-XL6
pCMV-XL4䇮pCMV-XL5䇮pCMV-XL6 䈲䈠䉏䈡䉏4.7kb䈫4.5kb䈱䉰䉟䉵䉕᦭䈜䉎䊔䉪䉺䊷䈪䈜䇯
ਔ䊔䉪䉺䊷౒䈮㩷Sac㸇䈎䉌㩷Sma㸇䈮⥋䉎䊘䊥䊥䊮䉦䊷㈩೉䉕᦭䈚䈩䈇䉁䈜䇯䊍䊃ቢో㐳㩷cDNA 䈲䈖
䉏䉌䊔䉪䉺䊷䈱㩷Eco R㸇䈫㩷Sal㸇䉰䉟䊃䈱㑆䈮䉪䊨䊷䊆䊮䉫䈘䉏䈩䈇䉁䈜䋨ૉ䈚㩷Sal 㸇䉰䉟䊃䈲䉪䊨䊷
䊆䊮䉫䈱䊒䊨䉶䉴䈪⎕უ䈘䉏䈩䈇䉁䈜䋩䇯
䋼ᧄ䊔䉪䉺䊷䈱․㐳䋾
䊒䊨䊝䊷䉺䊷㩷䋺㩷CMV䊒䊨䊝䊷䉺䊷
polyA䉲䉫䊅䊦㩷䋺㩷hGH(human growth hormone) polyA
䊋䉪䊁䊥䉝䈮䈍䈔䉎ⶄ⵾⿠ὐ㩷䋺㩷ColE1
⌀ᩭ↢‛䈍䈔䉎ⶄ⵾⿠ὐ㩷䋺㩷SV40 ori
䊐䉜䊷䉳䈍䈔䉎ⶄ⵾⿠ὐ㩷䋺㩷f1 ori
ㆬᛯ䊙䊷䉦䋨ᄢ⣺⩶䋩㩷䋺㩷䉝䊮䊏䉲䊥䊮
in vitro⊒⃻♽䊒䊨䊝䊷䉺䊷㩷䋺㩷T7䉁䈢䈲SP6䊒䊨䊝䊷䉺䊷
૶↪ᣇᴺ
Ԙࡊࡠ࠹ࠢ࠻ࡎ࡞࠳࡯߆ࠄࡊ࡜ࠬ࠴࠶ࠢߩኈེࠍขࠅߛߒߡਅߐ޿‫ޕ‬
ԙኈེࠍᷡẖߥࡔ߽ࠬߒߊߪࡂࠨࡒߢ㐿ኽߒߡਅߐ޿‫ޕ‬
ԚṌ⩶ߒߚࡇࡦ࠮࠶࠻ࠍ↪޿ኈེ߆ࠄࡈࠖ࡞࠲࡯ࠍขࠅߛߒ1.5mlߩ
ࠛ࠶ࡍࡦ࠼࡞ࡈ࠴ࡘ࡯ࡉߩౝ஥ߦ౉ࠇߡਅߐ޿‫ޕ‬
ԛ⬄ࠍ㐽߼‫ߪߚ߹ࡊ࠶࠻࠴ࡦࡌޔ‬ᐔࠄߥᚲߦ㈩⟎ߒߡਅߐ޿‫ޕ‬
Ԝࡈࠖ࡞࠲࡯߇࠴ࡘ࡯ࡉߩਛᔃߦሽ࿷ߔࠆࠃ߁ߦࡌࡦ࠴࠻࠶ࡊ਄ߢ
ࠁߞߊࠅߣំߔߞߡਅߐ޿㧔࿑2-1㧕‫ޕ‬
࿑2-1
ԝṌ⩶ߒߚ⣕ࠗࠝࡦ੤឵᳓30Ǵlࠍࡈࠖ࡞࠲࡯ߩਛᔃߦᷝടߒߡਅߐ޿㧔࿑2-2㧕‫ޕ‬
ࠢࡠ࡯ࡦࠍ฽߻ṁᶧߪࡈࠖ࡞࠲࡯ࠍㅢࠅ‫ߩࡉ࡯ࡘ࠴ޔ‬ოߣࡈࠖ࡞࠲࡯ߩ
㑆ߦ࿷࿷ߒ߹ߔ㧔࿑2-3㧕‫ޕ‬
࿑2-2
࿑2-3
Cross-section of
Eppendorf tube
Filter Paper
Area of eluant
Ԟ15Ǵlߩṁ಴‛ࠍขࠅṌ⩶ߒߚᣂߒ޿1.5mlߩࠛ࠶ࡍࡦ࠼࡞ࡈ࠴ࡘ࡯ࡉߦᷝടߒߡਅߐ޿‫ޕ‬
ԟࠦࡦࡇ࠹ࡦ࠻࠮࡞ࠍṁ಴‛ߦᷝടߒ‫ߡߒࡦ࡚ࠪ࡯ࡔࠜࡈࠬࡦ࡜࠻ޔ‬ਅߐ޿
㧔ṁ಴‛ߪPCRჇ᏷߽น⢻ߢߔ㧕‫ޕ‬
Ԡ࠻࡜ࡦࠬࡈࠜࡔ࡯࡚ࠪࡦߒߚ⚦⢩ߪLB-ampicilin(100mg/plate)਄ߢ37͠‫ߢ࠻ࠗ࠽ࡃ࡯ࠝޔ‬
ࠗࡦࠠࡘࡌ࡯࡚ࠪࡦߒߡਅߐ޿‫ޕ‬ฦ‫ߡోߪ࡯࠾ࡠࠦߩޘ‬หߓࡊ࡜ࠬࡒ࠼ࠍ฽ࠎߢ޿߹ߔ‫ޕ‬
࡮ࠦࡠ࠾࡯ߩ૶↪ߦߟ޿ߡ
‫ڎ‬2%4Ⴧ᏷˴
‫ࠢ࠶࠻ࠬ࡞࡯ࡠ࠮࡝ࠣڎ‬૞ᚑߩߚ߼ߦၭ࿾ߢၭ㙃
‫&࠼ࡒࠬ࡜ࡊڎ‬0#࿁෼ߩߚ߼ߦၭ࿾ߢၭ㙃
ᬌ⚝ᣇᴺ
OriGene␠䊍䊃ቢో㐳cDNA䈮㑐䈜䉎⵾ຠᬌ⚝䈮䈧䈇䈩
OriGene␠䊍䊃ቢో㐳cDNA䈮㑐䈜䉎⵾ຠᢙ䈲⚂20,000ຠ⋡ሽ࿷䈚䉁䈜䇯⊝᭽䈱Ꮧᦸ䈘䉏䉎⵾ຠត
⚝䈲OriGene␠Web䈎䉌ᬌ⚝䈜䉎ᔅⷐ䈏䈅䉍䉁䈜䇯એਅ䈮ᬌ⚝ᣇᴺ䈍䉋䈶䈗ᵈᢥ䈮ᔅⷐ䈭ᖱႎ䊶ᣇ
ᴺ䈮䈧䈇䈩➙䉄䉁䈚䈢䈱䈪䇮䈗⚫੺䈘䈞䈩㗂䈐䉁䈜䇯
䋼⵾ຠ䈱ሽ࿷䉕ᬌ⚝䈜䉎႐ว䋾
ԘOriGene␠䈱䉡䉢䊑䉰䉟䊃䋨http://www.origene.com䋩䈮䉝䉪䉶䉴䈚䈩ਅ䈘䈇䇯
ԙ䉰䉟䊃Ꮐ┵䈱"Search for clones by words in Description"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
Ԛ䉰䉟䊃Ꮐ┵䈱"Search and Order Human Genes"䉕ㆬᛯ䈚䈩ਅ䈘䈇䇯
ԛ䊌䉴䊪䊷䊄䈫䈭䉎䊜䊷䊦䉝䊄䊧䉴䉕⸥౉䈚䈩ਅ䈘䈇䇯
Ԝ"Continue"䉕䉪䊥䉾䉪䈜䉎䈫"Search and Order Human Genes"ᬌ⚝㐿ᆎ䈱↹㕙䈮ធ⛯䈘䉏䉁䈜䇯
䋼NM Number䈮䉋䉍⵾ຠ䈱ሽ࿷䉕ᬌ⚝䈜䉎䋾
Ԙ⊝᭽䈏NCBI䈱䊂䊷䉺䊔䊷䉴䈮䉋䉍ᬌ⚝䈚䈢䈇ㆮવሶ䈱NM Number䈮䈧䈇䈩ᖱႎ䉕
㩷㩷㩷㩷㩷଻᦭䈚䈩䈇䉎႐ว䇮↹㕙Ꮐ┵䈱"by NM or XM Number"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
ԙ↹㕙ਛᄩ䈮NM Number䉕ᛂ䈤ㄟ䉖䈪"search"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯⵾ຠ䈏ሽ࿷䈜䉎
㩷㩷㩷㩷㩷႐ว䇮Accession number䇮Description䇮OriGene Clone Number䈏ឝタ䈘䉏䈢↹㕙䈏⃻䉏䉁䈜䇯
଀㧦"NM Number"䈮014797䉕ᛂ䈤ㄟ䉖䈪"search"䉕䉪䊥䉾䉪䇯ᰴ↹㕙䈮䈲એਅ䈱䊂䊷䉺䈏
㩷㩷㩷㩷㩷㩷⸥タ䈘䉏䉁䈜䇯
Accession number : NM_014797
Description : Homo sapiens KIAA0441 gene product (KIAA0441), mRNA Size : 5597
OriGene Clone Number: AB3304_A04
䈗ᵈᢥ䈱㓙䈮䈲䈖䉏䉌䈱Accession number䇮Description䇮OriGene Clone Number䈮㑐䈜䉎
ᖱႎ䈏ᔅⷐ䈫䈭䉍䉁䈜䇯
䋼䉲䊷䉪䉣䊮䉴䊂䊷䉺䉕ၮ䈮䈚䈢⵾ຠ䈱ሽ࿷䉕ᬌ⚝䋾
Ԙ⊝᭽䈏ᬌ⚝䈚䈢䈇ㆮવሶ䈱䉲䊷䉪䉣䊮䉴䊂䊷䉺䈮ၮ䈨䈐䇮⵾ຠ䈱᦭ή䉕ᬌ⚝䈜䉎႐ว䇮↹㕙Ꮐ┵
㩷㩷㩷㩷㩷䈱"by Nucleotide Sequence"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
ԙ↹㕙ਛ䈱"Enter Sequence Data"䈮䉲䊷䉪䉣䊮䉴䊂䊷䉺䉕ᛂ䈤ㄟ䉖䈪ਅ䈘䈇䇯
଀㧦ᛂ䈤ㄟ䉃䉲䊷䉪䉣䊮䉴䊂䊷䉺㵘㵘㵘
˴˴IICIVEVVCICCEEECIIIEEEIECCIVEEVEIEIEIIEVIEEIVICVIECICCCVCEIC
˴˴ICCCEVIICICCICVVIIIICCIIECEEVCEIICCECIVIVVECCIIEECCCCCEEIIIC
˴˴ICEVECVICICVVIVIIEVEVICCCEIIIVICIIEVIICVICEICVICEICIIIVIVIEE
˴˴ICIVVEVIEEEVVEIIICICVEVIEEVCEVECCIICIEVICCIECECCICCECVEIVECI
˴˴IEVEECVICEIVEEVIECECIEICECCICCIEVICEIVVIIVVVVVICIVVEVIEICEEC
˴˴IICEEVVCCICCIVCVVVEICECIEVIECCVIIVICEEVCICVEEVICCCVVIVICCIVE
˴˴CVVEEVEVVEECIEVIEVICCCIIEEVIIIEVVEVIVECECIEEICCCEIVIEVIECECI
˴˴IICEEVICCIEEVECICCEEVIEVECVECCECIICCVIIIICIEVICCCEVIIEVICVVV
˴˴VIIVVVIIEVEIEIEEVVVIIICVEEEVIVVEIEVIVVCEVECIEVICIIVEIVECEIEV
˴˴IVIIVCEEIEEECEEVICVIVEEVEVVVIIIIEECCIEVIVCEVEECEIVEECVEICECV
˴˴IVIIVECIEVIIEVIECVEVVEIECICIEVIIEECCEIEVIIIEIIEEEEVEVVVEEVII
Ԛ"Submit BLAST query"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯ᰴ↹㕙䈮䈲એਅ䈱䊂䊷䉺䈏⸥タ䈘䉏䉁䈜䇯
5GSWGPEGURTQFWEKPIUKIPKHKECPVCNKIPOGPVU
DKVU8CNWG
0/A˴*QOQUCRKGPUE[ENKPFGRGPFGPVMKPCUG
%&
0/A*QOQUCRKGPUOKVQIGPCEVKXCVGFRTQVGKPMKPG
0/A*QOQUCRKGPUE[ENKPFGRGPFGPVMKPCUG
%&G
:/A*QOQUCRKGPUECNEKWOECNOQFWNKPFGRGPFGPVG
䈖䉏䉌䈱↹㕙䈲ᛂ䈤ㄟ䉖䈣䉲䊷䉪䉣䊮䉴䊂䊷䉺䈫⋧หᕈ䈱㜞䈇ㆮવሶ䈏೉᜼䈘䉏䈠䈱ㆮવሶฬ෸䈶
NM Number䈏೉᜼䈘䉏䈩䈇䉁䈜䇯⋡⊛䈫䈜䉎ㆮવሶฬ䈱䋨⋧หᕈ䈱ᦨ䉅㜞䈇䉅䈱䋩NM_䉕䉪䊥䉾䉪䈜
䉎䈫䈗ᵈᢥ䈮ᔅⷐ䈭Accession number䇮Description䇮OriGene Clone Number䈮㑐䈜䉎ᖱႎ䈏ឝタ
䈘䉏䉁䈜䇯
䋼䉺䊮䊌䉪⾰䈱䊄䊜䉟䊮䉕ၮ䈮䈚䈢⵾ຠ䈱ሽ࿷䉕ᬌ⚝䋾
Ԙ⊝᭽䈏䉺䊮䊌䉪⾰䈱䊄䊜䉟䊮ฬ䈮䉋䉍ᬌ⚝䈚䈢䈇႐ว䇮↹㕙Ꮐ┵䈱"by Domain"䉕䉪䊥䉾䉪䈚䈩
ਅ䈘䈇䇯
ԙ↹㕙ਛᄩ䈱"If you wish to view and print the complete list of domains, click here"䉕䉪䊥䉾䉪䈚䇮
䊄䊜䉟䊮䊥䉴䊃䉕ෳᾖ䈚䈩ਅ䈘䈇䇯
Ԛ䊥䉴䊃䈎䉌⋡⊛䈫䈜䉎䉺䊮䊌䉪⾰䈱䊄䊜䉟䊮ฬ䉕⏕⹺䈚䇮䈖䈱↹㕙䉕㐽䈛䈩ਅ䈘䈇䇯
ԛ⋡⊛䈫䈜䉎䉺䊮䊌䉪⾰䈱䊄䊜䉟䊮ฬ䉕"Find Domain Names Like"䈱䉦䊤䊛䈮ᛂ䈤ㄟ䉖䈣ᓟ䇮
"Find domain names"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
Ԝ↹㕙ਛᄩ䈱"Select One Domain from List (1): "䈎䉌⋡⊛䈫䈜䉎䉺䊮䊌䉪⾰䈱䊄䊜䉟䊮ฬ䉕ㆬᛯ䈚
"search"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯ᰴ↹㕙䈱"Return Result "䈱ਅㇱ䈮ㆬᛯ䈚䈢䊄䊜䉟䊮䉕฽䉃
OriGene␠଻᦭䈱⵾ຠ䈏⴫␜䈘䉏䉁䈜䇯
଀㧦䊄䊜䉟䊮ฬ䉕14-3-3䈫ᛂ䈤ㄟ䉂ᠲ૞䈚䈢႐ว
Return Result :
NM_003404 - NM_003405 - NM_003406 - NM_006142 - NM_006761 - NM_006826 NM_012479 ԝ਄⸥↹㕙ਛ䈱NM_䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯䈗ᵈᢥ䈮ᔅⷐ䈭Accession number䇮Description䇮㩷
OriGene Clone Number䈮㑐䈜䉎ᖱႎ䈏ឝタ䈘䉏䉁䈜䇯
䋼ౕ૕⊛䈭䉺䊮䊌䉪⾰ฬ䉕ၮ䈮䈚䈢⵾ຠ䈱ሽ࿷䉕ᬌ⚝䋾
Ԙ⊝᭽䈏䉺䊮䊌䉪⾰ฬ䉕䉨䊷䊪䊷䊄䈫䈚䈩⵾ຠ䈱᦭ή䉕ᬌ⚝䈜䉎႐ว䇮↹㕙Ꮐ┵䈱by Words in
description䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
ԙ"Search Text 1"䈮䉨䊷䊪䊷䊄䉕ᛂ䈤ㄟ䉂"Search"䉕䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯
Ԛㆬᛯ䈚䈢䊄䊜䉟䊮䉕฽䉃OriGene␠଻᦭䈱⵾ຠ䈏⴫␜䈘䉏䉁䈜䇯NM䌟䈏⴫␜䈘䉏䉁䈜䈱䈪䇮
䉪䊥䉾䉪䈚䈩ਅ䈘䈇䇯䈗ฃᵈ䈮ᔅⷐ䈭Accession number䇮Description䇮OriGene Clone Number䈮
㑐䈜䉎ᖱႎ䈏ឝタ䈘䉏䉁䈜䇯
ᵈᗧ੐㗄
䂓㩷OriGene␠䈱䊍䊃ో㐳䌣DNA䈮䈧䈇䈩䈱ᵈᗧ੐㗄
㵘OriGene␠䈱䊍䊃ో㐳䌣DNA䈲䇮㕖༡೑⊛䈭⎇ⓥ㐿⊒䈱⋡⊛䈮䈱䉂૶↪䈏⸵น䈘䉏䈩䈇䉁䈜䇯ᓥ䈇
䉁䈚䈩䇮䈖䈱⋡⊛䈪䈱䉂䈗૶↪ਅ䈘䈇䇯
㵘䊍䊃ో㐳䌣DNA䈱⊒⃻↢ᚑ‛䇮ᒰ⹥ో㐳䌣DNA䈱৻ㇱ䉕↪䈇䈢⊒⃻↢ᚑ‛䋨એਅ䇮䈖䉏䉌䉕䉁䈫䉄䈩䇸
ᒰ⹥⊒⃻↢ᚑ‛䇹䈫䈇䈇䉁䈜䋩䇮ਗ䈶䈮ᒰ⹥䊍䊃ో㐳䌣DNA䈱ోㇱ䈅䉎䈇䈲৻ㇱ䈱㈩೉䇮䉁䈢䈲䈖䉏䉌
䈱ᡷᄌ㈩೉䋨એਅ䇮䈖䉏䉌䉕䉁䈫䉄䈩䇸ᒰ⹥㈩೉䇹䈫䈇䈇䉁䈜䋩䈮䈧䈇䈩䈲䇮䈍ቴ᭽䈗⥄り䈏⹜㛎⎇ⓥ䈱
䈢䉄䈮䈗૶↪ਅ䈘䈇䇯
㵘એਅ䈮䈗⺑᣿䈜䉎䉋䈉䈮䇮䇸ᒰ⹥⊒⃻↢ᚑ‛䇹䈍䉋䈶䇸ᒰ⹥㈩೉䇹䉕䇮䈍ቴ᭽䈏༡೑⋡⊛䈪↢↥䉁䈢䈲
૶↪䈜䉎䇮䈅䉎䈇䈲ઁੱ䈮⼑ᷰ䈜䉎䈭䈬䈱ታᣉⴕὑ䈮䉋䉍䇮⍮⊛⽷↥ᮭ䈮䈎䈎䉎໧㗴䈏⊒↢䈚䈩䉅ᒰ
␠䈲৻ಾ䈱⽿છ䉕⽶䈇䉁䈞䉖䇯
㵘䇸ᒰ⹥⊒⃻↢ᚑ‛䇹䈮⍮⊛⽷↥ᮭ䈏ሽ࿷䈜䉎႐ว䇮䇸ᒰ⹥⊒⃻↢ᚑ‛䇹䈱ታᣉⴕὑ䋨↢↥䇮૶↪䇮⼑
ᷰ䋨⾉ᷰ䈚䉕฽䉂䇮᦭ఘήఘ䉕໧䉒䈭䈇䋩䇮⼑ᷰ䈱↳಴䈅䉎䈇䈲ዷ␜䈜䉎ⴕὑ䈭䈬䋩䈲ᒰ⹥ᮭ೑䉕ଚኂ
䈚䉁䈜䇯ᓥ䈦䈩䇮䈖䈱䉋䈉䈭ታᣉⴕὑ䉕ᗧ࿑䈘䉏䉎႐ว䈲䇮䇸ᒰ⹥⊒⃻↢ᚑ‛䇹䈮⍮⊛⽷↥ᮭ䈏ሽ࿷䈜
䉎䈎ุ䈎䉕䇮䈍ቴ᭽䈗⥄り䈪⺞ᩏ䈘䉏䇮ᒰ⹥ᮭ೑䉕ଚኂ䈚䈭䈇䉋䈉䈮䈗ᵈᗧਅ䈘䈇䇯ᒰ␠䈲䇸ᒰ⹥⊒⃻
↢ᚑ‛䇹䈍䉋䈶䇸ᒰ⹥㈩೉䇹䈮⍮⊛⽷↥ᮭ䈏ሽ࿷䈜䉎䈎ุ䈎䈮䈧䈇䈩䈱⺞ᩏ䉕ⴕ䈇䉁䈞䉖䈚䇮䇸ᒰ⹥⊒
⃻↢ᚑ‛䇹䈍䉋䈶䇸ᒰ⹥㈩೉䇹䈮⍮⊛⽷↥ᮭ䈏ሽ࿷䈚䈭䈇䈖䈫䉕଻⸽䈚䉁䈞䉖䇯
㵘䈭䈍䇮䇸ᒰ⹥⊒⃻↢ᚑ‛䇹䈲䇮䉺䊮䊌䉪⾰䇮ᩭ㉄䋨RNA䇮DNA䋩䈭䈬䇮䇸ᒰ⹥㈩೉䇹䈮↱᧪䈜䉎䈜䈼䈩䈱
↢ᚑ‛䈍䉋䈶䈠䈱ᡷᄌ‛䉕฽䉂䉁䈜䇯䉺䊮䊌䉪⾰䈫䈚䈩䈲䇮㉂⚛䇮᛫૕䇮᛫ේ䇮䊧䉶䊒䉺䊷䇮䊖䊦䊝䊮䈭
䈬䈏଀␜䈘䉏䉁䈜䈏䇮䈖䉏䉌䈮㒢ቯ䈘䉏䉁䈞䉖䇯
㵘䉁䈢䇮⍮⊛⽷↥ᮭ䈮䈲䇮․⸵ᮭ䋨䉮䊮䊏䊠䊷䉺䊷䊒䊨䉫䊤䊛䉕฽䉃䋩䇮ታ↪ᣂ᩺ᮭ䇮ᗧඅᮭ䇮໡ᮡᮭ䇮
⒳⧣ᴺ䈮ၮ䈨䈒ᮭ೑䇮⪺૞ᮭ䇮ਇᱜ┹੎㒐ᱛᴺ䈮ၮ䈨䈒ᮭ೑䈭䈬䈏฽䉁䉏䉁䈜䇯
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