Intramedullary and extramedullary B lymphopoiesis in
osteopetrotic mice
by Hisashi Tagaya, Takahiro Kunisada, Hidetoshi Yamazaki, Toshiyuki Yamane,
Takeshi Tokuhisa, Erwin F. Wagner, Tetsuo Sudo, Leonard D. Shultz, and Shin-Ichi
Hayashi
Blood
Volume 95(11):3363-3370
June 1, 2000
©2000 by American Society of Hematology
Extramedullary hematopoiesis in 8-week-old mi/miand Fos (−/−) mice.The numbers of colonyforming cells elicited by 100 U/mL IL-3 or 20 U/mL IL-7 per 105 cells from bone marrow, spleen,
liver, thymus, peripheral lymph nodes (cervical, axillary, and inguinal)...
Hisashi Tagaya et al. Blood 2000;95:3363-3370
©2000 by American Society of Hematology
Bone marrow from aged op/op mice in which osteopetrosis was spontaneously cured produced
CFU–IL-7.The numbers of CFU–IL-3 and CFU–IL-7 in 30-week-old op/ophomozygous and
heterozygous littermates were examined.
Hisashi Tagaya et al. Blood 2000;95:3363-3370
©2000 by American Society of Hematology
Presence of mature B cells in the mi/mi spleen.Spleen cells (106) from 8-week-old
mi/mihomozygotes or mi/+ normal littermates were stained with FITC-6B2 (anti-B220),
biotinylated-IgM or -M1/70 (anti-Mac-1), and PE-streptavidin, and analyzed using a flow cyt...
Hisashi Tagaya et al. Blood 2000;95:3363-3370
©2000 by American Society of Hematology
Expression of mRNA of Mgf, Flt3l, and Il7in the osteopetrotic mice.Total RNAs were prepared
from bone marrow, spleens, or livers of 8-week-old osteopetrotic mice.
Hisashi Tagaya et al. Blood 2000;95:3363-3370
©2000 by American Society of Hematology