Antioxidant activity of superoxide dismutase and glutathione

ANNALES
2010
12
!" #$%#&#'(")*" %+,)%+&"%-,% (#$./&%"
3
45
3
44
4
3
5
Antioxidant activity of superoxide dismutase and glutathione
peroxidase in cattle tissue
" (#6,()+(7"6 (8"#9:)(,!. ;(#") &"6#87%#6,();('&. %#"#87
8 6"6+=/()>
Bovine cysticer+#,%,%").+)/(Taenia saginata%,;##"# %+)%,,8" %"'#. %"%",+ %#"
!,., $# = #, !# ! ) + %#" #$ +(, %++% ?+(, ,@ /+., %"$+ ) + & ,#.+ #$
=.!"%"$+ %#"%$$$+ )/$%, "8#.")+##6)CDFDGH" =(IIJKM8=,%"
IIMOP%" , %"&+, #)%"$+ %#",+.,)/( =%, 8#!,8'%, )%"#&")CDDDMDOH
".#Taenia saginata%,, %&&," ")%"+.+#"#!%+&#,,). #+#")!" %#"$%' %#"
"))#8"')%"'#$%"$+ ),&.'= + &CMHTaenia saginata%"=/% , = %,,.,#$+ &).%"'% ,
&-&, '")%"$+ , =%" , %"#$=.!",%"% ,).& $#!Cysticerci=-/",=#8" #Q%, %"
=&(!= %+-,,&,#$ == ,6& &!.,+&")&%-CDJH
=,% %"$+ %#"+.,,+ %- %#"#$ ==#, %!!.",(, !+#!#"" ,CDPH
+. #
+=#"%+ %,,. )!' =, /" ),+%/) %" = +&&, #$ =#, , %"$+ ) 8% = )%$$" ,+%, #$
,% ,). # =%"+,)!#." #$+ %-#Q('")%+&,8=%+=+.,&%%)#Q%) %#"CJH
#).+ ,#$&%%)#Q%) %#"?$)%+&,@$#!)%"-%#.,/%#+=!%+&+ %#","#!&&(
$$+ %-&(,+-"')/(" %#Q%)" , = &,#,.,, =+ %#",#$+ %-#Q('",./, "+,
" %#Q%)" )$"+, %"+&.) /# = ";(! %+ ") "#"";(! %+ ,(, !, .#Q%) )%,!. ,
'&. =%#"#Q%),")+ &, =%"+%&";(! %+" %#Q%)" ,=";(! %+$+ #,
).+)'&. =%#"-% !%"-% !%"R+# "+.&#&,!%"")/%&%./%"8=%+=!(
$."+ %#"," %#Q%)" ,CPDIH
=%, ##.6"#8&)'"#, .)(8=%+=-&. , =" %#Q%)" ,, .,%" =(, %++#,%,
=$# =%!#$ =%,%, #,,,, =+ %-% %,#$")*Q%" =+ & %,,.,%"$+ )
8% =Taenia saginata.
U
" =%,, .)(,.#Q%))%,!. ,")'&. =%#"#Q%),+ %-% (8,%"-, %' )%" =
,6& &!.,+&, 6"$#!GI+ &++,,," .&&(%"$+ )8% =Taenia saginata")J=& =(
82
3;(!#8,63.,;+;64%7#> (,
=),#$+ &?$#! W+ #(X! Y%".6#8#&")@= %,,.,!&,?D'!+=@
8 8,=) %" =#,= /.$$) ,&%" =#!#'"%;) %" GI ! %,U& D ! Z U
JO /.$$ P0 ") +" %$.') ( .,%"' ")#Q 6% , ?
*&. =%#" #Q%), ")
.#Q%)%,!. ,@ =+ %-% %,#$ ")*Q%" =,." " ,#$!.,+&
%,,.=#!#'" ,?I!'[!&@8) !%")
"DI\,#)%.!)#)+(&,.&$ ?@#&(+(&!%)'&&+ #=#,%, =# %",!&,
?GI]'@$#!&(, ,8, )") ",$) #"% #+&&.&#,!!/",?%&&%#@=
!!/", 8 /&#+6) 8% = D\ )$ ) !%&6 %" /.$$ U MG 8% = ID\ % #" DII
") ="Q#,) #+#!!+%&&(-%&/&%!(" %/#)%,?*&. =%#"#Q%),D" %/#)(
/+!Z .[4" " %/#)( *"Q@ =" = !!/", 8 %"+./ ) 8% = ,+#")(
" %/#)%,+#"7.' )8% =&6&%"=#,= ,?%'!@$# 8#=#., ##! ! .
(+ %#"#$?"% #/&. ;#&%.!@")?G/#!#P+=&##F%")#&(&=#,= @
%"/.$$?DII!%,U&UKGDII!&@ =/"),8-%,.&%;)=/")%" ",% %,
8+#!)8% = =+#" #&#",
RESULTS
.,.& ,,=#8) = =+ %-% (#$ =")*Q!,.),+ #=# #! %+&&(8,
%"+,) %" ,6& & !.,+& %,,., #$ ,&.'= %"$+ ) + & + %-% ( 8, #- 8# $#&)
=%'= ? DGJDG ['Z aIIIIIF@ ?W%' D@ 8=" +#!) 8% = = + %-% ( #$ =%, ";(!
%" = +#" #& '#. ? MJDII ['@ 8=, '&. =%#" #Q%), + %-% ( %" %,,., #$ =
"%!&,%"$+ )Taenia saginata8,=%'=?DFMFI['ZaIIIIO@%"+#!%,#"8% = =, .)%)
'#.#$=& =("%!&,?GPKO['@?W%'@
25000
SOD [U/g]
20000
control
15612.5 *
skeletal muscles infected
with T. saginata
15000
10000
7610
5000
0
*p<0.05
W%'D#!%,#"#$,.#Q%))%,!. ,?@+ %-% (%",6& &!.,+&,#$ =+#" #&
and Taenia saginata%"$+ )+ & %,,.,
" %#Q%)" + %-% (#$,.#Q%))%,!. ,")'&. =%#"#Q%),%"+ & %,,. OF
2000
1800
GPx [U/g]
1600
1373.04 *
control
skeletal muscles infected
with T. saginata
1400
1200
1000
800
549.82
600
400
200
0
*p<0.05
W%'#!%,#"#$'&. =%#"#Q%),?*Q@+ %-% (%",6& &!.,+&,#$ =+#" #&
and Taenia saginata%"$+ )+ & %,,.,
, "/&# %,,.Q + ,"&(,%,) + )DJ6/")$#,.#Q%))%,!. ,8=%+=
+#,#")) 8% = = -&. #$ ,%"'& !#"#! .4" ") % , + %-% ( 8, +#!) 8% =
, ,!&,%"$+ )8% = 8#!,,(" =,%,, %!.& %#")%" ==#, %,,.,
+#" %"%"'+(, %++.,%"+#" , 8% = =+#" #&,!&,?W%'F@='&. =%#"#Q%),
,# %"/")8% = =!#&+.&!,,!#." %"' #68,%)" %„)?W%'P@=8
"#,%'"%„+" )%$$"+,%" ='&. =%#"#Q%),/")%" ",% %,#/,-) =%" =, .)%)
,!&,%"+#!%,#"8% = =+#" #&"%!&,
W%'FQ!&,#$%!!."#/&# + %#" ",#$," %-,6& &!.,+&,$#!+ &
%"$+ )/(Taenia saginata+#!) # ==& =("%!&,# %"8,$#.") #=-
!#&+.&,%;#$DJ6
&"DJ+#" #&,Z&"DDI%"$+ )!.,+&,!&,
OP
3;(!#8,63.,;+;64%7#> (,
A
W%'PQ!&,#$ =%!!."#/&# + %#" ",#$," %-,6& &!.,+&,$#!
+ &%"$+ )/(Taenia saginata+#!) # ==& =("%!&,*Q# %"8,$#.") #=-
!#&+.&,%;#$6
&"DJ+#" #&,Z&"DDI%"$+ )!.,+&,!&,
= „, , ).%"' %"$+ %#" #$ = ,% %, + %#" #$ = =#, #'"%,! /( + %- %"'
+#!&!" )")%"' %!!." ,#", CDH U%'=&( + %- #Q('" ,+%, ?2.- U22 and –
U@'" )/(+ %- )='#+( ,%"+&.)%"'#,%"#=%&,")". #=%&,," #8$.&
$$+ #!+="%,!'%", ,% ,CDFODPH
.#Q%))%,!. ,")'&. =%#"#Q%),/&#"' # =!%")$"," %#Q%)" , = -" =$#! %#"#$"8$)%+&,+%,=(+#"- =Q%, %"'$)%+&,%" #&,,
=!$.& !#&+.&, /$# =( =- +="+ # + +#"- , ,.#Q%) %" # =()#'"
#Q%)")*Q+="',=()#'"#Q%)%" #=!&,,!#&+.&,=., =(-" ,.#Q%)
") =()#'" #Q%) $#! %" + %"' ") #).+%"' =()#Q(& ") ,%"'& #Q('" =#.'= W
)")" W" #"+ %#"CJH
" =," , .)(8#/,-), %, %+&&(,%'"%„+" %"+,?& %- # =+#" #&-&.
%" ."%"$+ ) + &@ %" = + %-% ( #$ " %#Q%)" ?,+ #=# #! %+&&( !,.) ") /(
8, " /&# +="%’.@ ") *Q ";(!, ?,+ #=# #! %+&&( !,.)@ %" ,6& & !.,+&,
%"$+ )/(Taenia saginata ?W%'DF@=8#/,-)"#,%'"%„+" )%$$"+,%" =+ %-% (
#$'&. =%#"#Q%),%" =Q!%"),!&,$#!+ &!.,+&,")%"+#" #&'#.,$#.")
.,%"' =8, "/&# +="%’.?W%'P@
" ",%„+ %#"#$ =" %#Q%)" #+,,,+#.&)/& ) # =,"+#$Taenia saginata
&-8=%+=!(%").+='#+( , #'" $)%+&,CJH=,,.& ,,.# )/(
# =, .)%,%")%+ %"' = ,.+=" %#Q%)" ";(!,,")*Q$."+ %#"%"',)$"+
!+="%,!'%", #Q('")%-)$)%+&,!(&("%!# " #&%" ==#, “,/%#+=!%+&
)$"+!+="%,!,).%"'+(, %++#,%,CGJKDH
=,.& ,," )=&)., #+#"+&.) = =%!/&"+/ 8"#")" %#Q%)" #+,,,&(!%"#&%" = =#&#'(,,#+% )8% =+(, %++#,%,=%"+,%"+ %-% (
" %#Q%)" + %-% (#$,.#Q%))%,!. ,")'&. =%#"#Q%),%"+ & %,,.
OG
#$ =" %#Q%)" ";(!,%" %,,.,#$%"$+ )"%!&,%,,#", ##Q%)%;%"', ,,8=%+=+"
/+.,)/( =,"+#$ =,% %""%!&,!.,+&,=+="',#/,-)%" =+ %-% (
#$" %#Q%)" ";(!,%" = %,,.,%"$+ )8% = 8#!&-+"=& #)-&#"8")
$$+ %-! =#),#$) + %"'!,&,%",&.'= "%!&,
"+, %" = + %-% ( #$ " %#Q%)" ";(!, %" %,,., #$ %"$+ )"%!&, %, ,#", #
#Q%)%;%"' , ,, 8=%+= +" / +.,) /( = ,"+ #$ = ,% %" "%!&, !.,+&, =,
,.& ,,.# =,%'"%„+"+#$" %#Q%)" #+,,,%"=#, )$",!+="%,!).%"',% %+
%"$+ %#",=.,% %,, ) = ,&.'= 8, ,"%!&,8% =) + )!,&,+#", % . -(
%!# " &!" #$„'= %"'8% =+(, %++#,%,") "%,%,##- =+="',#/,-)%" =
+ %-% (#$" %#Q%)" ";(!,%" = %,,.,%"$+ )/( 8#!&-+"=& #)-&#"8
")$$+ %-! =#),#$) + %"'!,&,%",&.'= "%!&,
REFERENCES
D&%",6#&"#)"%6%8%"8;7%,#•( "%+;7%);( #&FODKK
#+;#"U),").,6#86)
, %!.& %#"#$" %#Q%)" ,%"!.,+&#$
Trichinella spiralis%"$+ ) ,
+ ,% #&PDDFODKKJ
F=%/&
&/#.%#%
&$$+ #$+(, %++.,+&.&#,#"". #=%&
$."+ %#"")) = ,% #&DMDKIIG
P'5 6%"
' U%%)#Q%) %#"")
" %#Q%)" # " %&#$=%-
"$+ ) .&&(8% =%, #! #,%,–6%(;% #&#7%'%,%FFJIIO
G ) U),  " %#Q%)" , ") # #&( %+ ";(!, %" Q%!" & %+=%"&&#,%, + ,% #&PGFJDIII
J)").,6#86U),="',%" =&-&#$" %#Q%)" ,%" =/&##)$#!!%+
%"$+ )8% =Trichinella spiralis,% #&,KFDIIIP
M#"( Taenia saginata%".# ,% #&DPKPIIM
OU"6&3 &=+ %; %#"")!#&+.&+&#"%"'#$.[4",.#Q%))%,!. ,$#! =
=.!",% Onchocerca volvulus"$+ !!."GKIJFIJKDKKD
K#&#);%7+;(6%!%"%.66;()&8,6Fasciola hepatica$$+ ,#" =" %#Q%) %-
# %,")&%%)#Q%) %#"#$ ,.!Q,% #&DDFPOIIJ
DI#;>#8,6—73;(!#8,63Q,,%#"#$,.#Q%))%,!. ,?@'"%"/&##)+&&,")
= #+( ,#$%" !)% =#, ,%"$+ )8% =Echinococcus granulosus (Batsch,1786). Annales
,+ DKDGFPKIIJ
DD#;>#8,6—73;(!#8,63-&"+#$+(, %++#,%,%"+ &")%',%" =./&%"#-%"+
%" =(,DKKFIIP
""&,,+ JDDMDPFIIJ
D %&%: & + %-% ( #$ " %#Q%) %- )$", ";(!, %" = /&##) #$ %" , 8% = &%-
+=%"#+#++#,%,#7"#,"% '&JPFGPIIIM
DF'."!%"7!%"3-&#!" ")„&)-&. %#"#$"8,#&#'%+& , $#"%
saginata cysticercosis. ,% #&DJKKFDIDIIK
86
3;(!#8,63.,;+;64%7#> (,
DP .'%" W% W % & $$+ #$ +(, %++., +&&.&#, $+ %#", #" =
,% #(/., #$%'". #=%&,-", )#&#.&#PMKPIIG
DG+") &%, %/. %#"#$Taenia saginata+(, %++%%" %,,.,#$Q%!" &&(%"$+ )
+ & ,% #&DJPFFDIIK
DJ˜ ™/3(6#-š +="%+6 %,,. + %#" %" == #$+ &8% =,#" "#.,")
%„+%&cysticercus bovis%"$+ %#"Folia Parasitol. (Praha),JFFDKMK
DM&#+=, #)%"$+ %#",%"#&")%"IIJ;'&%)!%#&JFGGIIO
DO&#+=, #)%"$+ %#",%"#&")%"IIM;'&%)!%#&JFJKIIK
5
" =%,, .)( =+ %-% (#$ 8#" %#Q%)" ";(!,,.#Q%))%,!. ,?DDGDD@")
'&. =%#" #Q%), ? DDDDK@ 8, Q!%") %" ,6& & !.,+&, $#! + & %"$+ ) 8% =
Taenia saginata #/,-) , %, %+&&( ,%'"%„+" %"+, ?& %- # = +#" #& -&. %"
."%"$+ ) + &@ %" = &-& #$ ";(!, %" ,+ #=# #! %+ "&(,%, ?,.#Q%) )%,!. ,
'&. =%#"#Q%),@")8, "/&# +="%’.?,.#Q%))%,!. ,@=8"#,%'"%„+" )%$$"+,%" =+ %-% (#$*Q%"+ & %,,.%"$+ )8% =+(, %++.,%"+#!%,#"8% = =
+#" #&=& =("%!&,,,,,)8% =8, "/&# +="%’.
.,.& ,,.'', = ")*Q";(!,%!# " %" ==#, “,/%#+=!%+&)$"+
!+="%,!,).%"'+(, %++#,%,
(8#), ,.#Q%))%,!. , '&. =%#"#Q%),Taenia saginata" %#Q%)" + &
44
4/)"# 6 (8"#9: )(,!. ;( #") &"6#87 ?@ % #6,();( '&. %#"#87 ?*Q@
8!%›9"%+=,;6%& #8(+=/()>;•#"'#Taenia saginata.4#!#+œ"&%;(,6 #$# #! (+;"7
;#/,8#8"# , (, (+;"% ;"+;œ+( 8;#, #;%#!. #/. ";(!—8 8 #—8""%. ; 6#" #&œ ;8%;› !% ;)#8(!% ;"+;"% #;%#!. " (#6,()" —8 ;#8);#" ! #)œ western
blot8(6;>#%, # "%8(•,;œ6 (8"#9:)(,!. ;(#") &"6#87./()>;"'#8, #,."6.
)# ;8%;œ "%;•#"(+= Taenia saginata. % , 8%);#"# ;"+;œ+(+= —•"%+ 8 #;%#!%
6 (8"#9+%#6,();('&. %#"#87;#, 6 (8"#9+%)(,!. ;(#") &"6#87%#6,();(
'&. %#"#87 8 6"6+= ;8%;œ ;•#"(+= 7, #)#8%);%œ " , , #6,()+(7"( 8(8#>"(
#/+"#9+%œ ,#•( 8 !%›9"%+= ;8%;œ #;""% ;!%" 6 (8"#9+% (+= ";(!—8 !#• /(:
%, # "88(6(8"%.8œ';(+(;8%;œ 6•7, "%;!%"%8•"(!&!" !#„&6 (6%
,%!+;(+(.&.);%
>#8 6&.+;#8 )(,!. ; #") &"6#8 #6,(); '&. %#"#8 Taenia saginata
" (#6,()" /()>#