Well-known published Tissue Culture Media (in date order)
Provided by Ronald de Fossard – April 2011
It is assumed that anyone mixing their own media can identify
the compounds from their chemical formulas.
KNOP'S (1865) MINERALS
Compound
Ca(NO3)2.4H2O
Mass per litre
1000 mg
KNO3
250 mg
MnSO4.4H20
250 mg
KH2PO4
250 mg
W-MINERALS: WHITE'S (1943) MEDIUM
Compound
Ca(NO3)2
Mass per litre
200 mg
KNO3
80 mg
KCl
65 mg
MgSO4
360 mg
NaH2PO4.H2O
16.5 mg
MnSO4
4.5 mg
ZnSO4
1.5 mg
KI
0.75 mg
Fe2(SO4)3
2.5 mg
Na2SO4
200 mg
KNUDSON (1946) MINERALS
Compound
Mass per litre
(NH4)2SO4
500 mg
MgSO4.7H2O
250 mg
MnSO4.4H20
7.5 mg
KH2PO4
250 mg
Ca(NO3)2.4H2O
FeSO4.7H2O
1000 mg
25 mg
VW-MINERALS: VACIN & WENT (1949) MEDIUM
Compound
Mass per litre
(NH4)2SO4
500 mg
MgSO4.7H2O
250 mg
KNO3
525 mg
MnSO4.4H20
7.5 mg
KH2PO4
250 mg
Ca3(PO4)2
200 mg
Fe2(C4H4O6).3H2O
28 mg
N-MINERALS: NITSCH'S (1951) MEDIUM
Compound
Mass per litre
Ca(NO3)2.4H2O
500 mg
MgSO4.7H2O
125 mg
KNO3
125 mg
KH2PO4
125 mg
H3BO3
0.5 mg
CuSO4.5H2O
0.025 mg
MnSO4.4H20
3 mg
Na2MoO4.2H2O
0.025 mg
ZnSO4.7H20
0.5 mg
H2SO4 (36N)
0.5 μL
FeC6H5O7.5H2O
10 mg
H-MINERALS: HELLER (1953) MEDIUM
Compound
Mass per litre
CaCl2.2H2O
75 mg
KCl
750 mg
MgSO4.7H2O
250 mg
NaNO3
600 mg
NaH2PO4.H2O
125 mg
H3BO3
1 mg
CuSO4.5H2O
0.03 mg
MnSO4.4H20
0.1 mg
KI
0.01 mg
ZnSO4.7H20
1 mg
AlCl3.6H2O
0.03 mg
NiCl2.6H2O
0.03 mg
FeCl3.6H2O
1 mg
MS-MINERALS: MURASHIGE & SKOOG (1962) MEDIUM
Compound
Mass per litre
NH4NO3
1650 mg
KNO3
1900 mg
CaCl2.2H2O
440 mg
MgSO4.7H2O
370 mg
KH2PO4
170 mg
H3BO3
6.2 mg
MnSO4.4H2O
22.3 mg
ZnSO4.7H20
8.6 mg
KI
0.83 mg
Na2MoO4.2H2O
0.25 mg
CuSO4.5H2O
0.025 mg
CoCl2.6H2O
0.025 mg
FeSO4.7H2O
27.8 mg
Na2EDTA.2H2O
37.3 mg
WIMBER-MINERALS: WIMBER (1963) MEDIUM
Compound
(NH4)2SO4
Mass per litre
500 mg
MgSO4.7H2O
250 mg
KNO 3
525 mg
KH2PO4
250 mg
CaHPO4.2H2O
200 mg
FeC4H4O6.H2O
30 mg
MM-MINERALS: MOREL & MULLER (1964) MEDIUM
Compound
Mass per litre
(NH4)2SO4
1000 mg
MgSO4.7H2O
KCl
Ca(NO3)2.4H2O
125 mg
1000 mg
500 mg
KH2PO4
125 mg
B5-MINERALS: GAMBORG, MILLER & OJIMA (1968)
Compound
Mass per litre
(NH4)2SO4
134 mg
CaCl2.2H2O
150 mg
MgSO4.7H2O
250 mg
KNO 3
2500 mg
NaH2PO4.H2O
150 mg
H3BO3
3 mg
CoCl2.6H2O
0.025 mg
CuSO4.5H2O
0.025 mg
MnSO4.H20
10 mg
KI
Na2MoO4.2H2O
0.75 mg
0.25 mg
ZnSO4.7H20
*SEQUESTRENE 330 Fe
2 mg
28 mg
NN-MINERALS: NITSCH & NITSCH (1969) MEDIUM
Compound
Mass per litre
NH4NO3
720 mg
KNO 3
950 mg
CaCl2
166 mg
MgSO4.7H2O
185 mg
KH2PO4
68 mg
H3BO3
10 mg
MnSO4.4H20
25 mg
ZnSO4.7H20
10 mg
Na2MoO4.2H2O
0.25 mg
CuSO4.5H2O
FeSO4.7H2O
0.025 mg
27.85 mg
Na2EDTA.2H2O
37.25 mg
SH-MINERALS: SCHENK & HILDEBRANDT (1972)
Compound
Mass per litre
NH4H2PO4
300 mg
CaCl2.2H2O
200 mg
MgSO4.7H2O
400 mg
KNO 3
2500 mg
H3BO3
5 mg
CoCl2.6H2O
0.1 mg
CuSO4.5H2O
0.2 mg
MnSO4.H20
KI
Na2MoO4.2H2O
10 mg
1mg
0.1 mg
ZnSO4.7H20
1 mg
FeSO4.7H2O
15 mg
Na2EDTA.2H2O
20 mg
WPM-MINERALS: LLOYD & McCOWN (1980)
Compound
NH4NO3
Mass per litre
400 mg
CaCl2.2H2O
96 mg
Ca(NO3)2.4H2O
556 mg
MgSO4.7H2O
370 mg
KH2PO4
370 mg
K2SO4
990 mg
H3BO3
6.2 mg
CuSO4.5H2O
0.25 mg
MnSO4.4H20
22.3 mg
Na2MoO4.2H2O
0.25 mg
ZnSO4.7H20
8.6 mg
FeSO4.7H2O
27.8 mg
Na2EDTA.2H2O
37.3 mg
DK-MINERALS: DRIVER & KUNIYUKI (1984) MEDIUM
Compound
NH4NO3
Mass per litre
1416.9 mg
CaCl2.2H2O
147.0 mg
Ca(NO3)2.4H2O
1960.1 mg
MgSO4.7H2O
739.5 mg
KH2PO4
258.6 mg
K2SO4
1227.8 mg
H3BO3
4.8 mg
CuSO4.5H2O
0.2 mg
MnSO4.4H20
44.6 mg
Na2MoO4.2H2O
0.4 mg
Zn(NO3)2.6H20
17.0 mg
FeSO4.7H2O
33.4 mg
Na2EDTA.2H2O
44.7 mg