GE Healthcare
Data File 18-1170-20 AC
Membrane separations
MidJet laboratory scale
membrane separations system
The MidJet™ laboratory scale membrane separations system
enables you to separate, concentrate, and diafilter small
volumes (up to 200-ml) of biological solutions. The system
uses hollow fiber cartridges for separation processing that is
easy and fast compared to other separations techniques. In
addition, when used with GE Healthcare hollow fiber
cartridges, you can scale your laboratory data linearly to
pilot- and production-scale systems.
Basic and advanced MidJet systems
The basic MidJet system includes a peristaltic pump,
reservoirs, tubing, fittings, backpressure valve, and a stand to
mount the system components. The advanced MidJet system
is comprised of a basic MidJet system plus pressure
transducers and displays for precise pressure and flow
control. Precise process control ensures high product recovery
and minimal shear denaturation, and provides data for scale
up to trials.
MidGee and MidGee Hoop cartridges
The MidJet system is perfectly matched to our entire line of
MidGee™ and MidGee Hoop™ membrane separations
cartridges. MidGee cartridges are available in a wide range of
ultrafiltration and microfiltration pore sizes for a variety of
laboratory, scale up and scale down applications.
Figure 1. Advanced MidJet system comes complete with peristaltic pump,
pressure transducers, and dual digital panel meter. Optional autoclavable
reservoirs are shown.
MidJet system features
MidGee cartridge specification
• Rapid processing of volumes up to 200 ml
Diameter:
0.3 cm (0.125 in.)
Length:
30.8 cm (12.125 in.)
End fitting connections:
Male Luer-Lok
Permeate connection:
Male Luer-Lok
Membrane area (nominal):
16 to 26 cm2 (2.32 -4.03 in2)
Hold-up volume (nominal):
Lumen side:
Shell side:
0.25 ml
0.25 ml
Autoclavable:
All except 1,000 NMWC models
Materials of construction:
Hollow fibers:
Housing components:
Polysulfone
Polysulfone/Polycarbonate
• Quick and easy cartridge change out using
Luer-Lok™ fittings
• Low system hold-up volume for concentration down
to 2-5 ml (cartridge hold-up volume of 0.5 ml)
• Easy scale-up to pilot and process volumes
• Perfect sizing for GE Healthcare MidGee and
MidGee Hoop hollow fiber cartridges
• Attaches to syringe for easy removal of retentate
• UF pore sizes from 1,000 to 750,000 NMWC*
• MF pore sizes from 0.1 to 0.65 micron
• Membrane area ranges from 16 to 73 cm2 depending
on lumen ID and fiber path length
• Optional autoclavable reservoirs and a three-port
pressure transducer with a temperature probe are
available
*Nominal molecular weight cutoff
Typical applications
• Monoclonal antibody clarification/concentration
• Plasmid concentration/diafiltration
• Protein concentration
• Suspended solids removal
• Vaccine concentration/purification
• Virus concentration/purification/removal
• Bacteria/pyrogen removal
• Plasma purification/concentration
MidGee cartridge selection
Ultrafiltration
Membrane fiber inner
diameter
Nominal molecular
weight cutoff
0.5 mm
0.5 mm
0.25, 0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
1 mm
1,000
3,000
10,000
30,000
50,000
100,000
300,000
500,000
750,000
Microfiltration
Membrane fiber inner
diameter
Pore
size
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
0.75 mm
0.1 micron
0.2 micron
0.45 micron
0.65 micron
MidGee cartridges are packaged in quantities of 6, 12 and 24. Single units
are available for trial orders.
• Buffer depyrogenation
• Cell harvesting/clarification
• Colloidal suspension concentration/diafiltration
• Continuous buffer exchange
• Continuous cell culture perfusion
• Hybridoma cell culture clarification
• Liposome purification
• Lysate clarification
• Mammalian cell clarification
Figure 2. MidJet Systems are designed for operation with both our
standard MidGee cartridges (right) and MidGee Hoop cartridges (left).
MidGee Hoop cartridges are offered in both 60 cm and 110 cm path
lengths to provide direct scale-up/scale-down from GE Healthcare pilot
and process scale units.
2
Data File 18-1170-20 AC
MidGee Hoop cartridge specifications
MidGee Hoop cartridge selection
Diameter:
0.3 cm (0.125 in.)
Ultrafiltration
Length:
Size H22 and H24
Size H42
60 cm (23.6 in.)
110 cm (43.30 in.)
End fitting connections:
Male Luer-Lok
Permeate connection:
Male Luer-Lok
Membrane area (nominal):
29 to 73 cm2 ( 4.490-11.31 in2)
Hold-up volume (nominal):
Size H22 and H24
0.5 ml shell side
Size H42
1.0 ml shell side
Autoclavable:
Yes
Materials of construction:
Hollow fibers:
Housing components:
Polysulfone
Polysulfone/Polycarbonate
0.5 to 1.0 ml lumen side
Membrane fiber inner
diameter
Nominal molecular
weight cutoff
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
1 mm
0.5, 1 mm
0.5, 1 mm
0.5, 1 mm
1 mm
3,000
10,000
30,000
50,000
100,000
300,000
500,000
750,000
0.6 to 2.0 ml lumen side
Microfiltration
Membrane fiber inner
diameter
Pore
size
1 mm
1 mm
1 mm
0.75 mm
0.1 micron
0.2 micron
0.45 micron
0.65 micron
MidJet system and accessories ordering information
Code number
Model number
Description
Code number
Model number
56-4106-10
PRT-DPM-2
Two pressure transducers with
digital panel meter readout
56-4105-87
56-4106-11
PRT-DPM-3T
Three pressure transducers and
temperature probe with digital
panel meter readout
56-4106-16
PTSL02-10
Recirculation pump tubing,
silicone, size 14, 1.6 mm (0.06 in)
ID, 3.1 m (10 ft)
KMDG-175R02A Autoclavable MidGee reservoir
replacement kit. Includes: (6)
polycarbonate reservoirs, 175 ml
capacity, conical bottom; (3) solid
reservoir caps; (6) caps with three
Luer ports; silicone tubing
Note: Kit KMDG-175R01A
required for reservoir bases
56-4106-17
PTSL03-10
Recirculation pump tubing,
silicone, size 16, 3.1 mm (0.12 in)
ID, 3.1 m (10 ft)
56-4106-15
PT-109-02
Replacement pressure
transducers (set of two)
Note: Requires calibration
56-4105-75
RBFL-1
Female luer to 1/8-in barb;
nylon, pkg of 10
56-4106-37
MDG-3SP
56-4105-76
RBFL-2
Female luer to 3/32-in barb;
nylon, pkg of 10
56-4105-83
KMRV-030
MidGee reservoir kit, 30 ml
capacity, includes: (12) each
reservoirs and caps; (2) sets cap
fittings; (12) sets reservoir internal
tubing
56-4105-84
KMRV-120
MidGee reservoir kit, 120 ml
capacity, includes: (12) each
reservoirs and caps; ( 2) sets cap
fittings; (12) sets reservoir internal
tubing
Basic MidJet system, includes:
miniature peristaltic pump* with
plug-in power supply;
exchangeable saddles for size 14
and 16 tubing; mounting
platform**; (6) 30 ml reservoirs,
caps, internal tubing sets; (12)
120 ml reservoirs, caps, internal
tubing sets; (4) sets cap fittings;
(1) backpressure valve; (10) Luer
to 1/8-in tubing adaptors; (10)
Luer to 3/32-in tubing adaptors;
silicone tubing, sizes 14 and 16,
1.5 m each
56-4106-38
MDG-4SP
Advanced MidJet system, same
as MDG-3SP plus: PRT-DPM-2
pressure transducers and digital
panel meter readout
56-4105-86
KMDG-175R01A Autoclavable MidGee reservoir
kit, includes: (3) polycarbonate
reservoirs, 175 ml capacity,
conical bottom; (3) solid reservoir
caps; (3) caps with Luer ports; (3)
reservoir bases to fit MidJet
system; (1) standard reservoir
base
Description
*Maximum nominal recirculation rate: 50 ml/min with size 14 tubing;
140 ml/min with size 16 tubing. Pump not sold separately.
**Mounting platform not sold separately.
Data File 18-1170-20 AC
3
Figure 3. MidGee cartridge showing configuration and Luer-Lok connectors
Figure 4. MidGee Hoop cartridge showing configuration and
Luer-Lok connectors
www.gehealthcare.com
Global Headquarters GE Healthcare
Little Chalfont
Buckinghamshire, U.K. HP7 9NA
GE, imagination at work and GE monogram are trademarks of
General Electric Company. MidJet, MidGee, and Hoop are
trademarks of GE Healthcare companies.
Luer-Lok is a trademark of Becton, Dickinson and Company.
All goods and services are sold subject to the terms and conditions
of sale of the company within GE Healthcare which supplies them.
GE Healthcare reserves the right, subject to any regulatory and
contractual approval, if required, to make changes in specifications
and features shown herein, or discontinue the product described
at any time without notice or obligation.
© 2006 General Electric Company - All rights reserved.
GE Healthcare Bio-Sciences AB, a General Electric Company.
GE Healthcare Bio-Sciences AB
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GE Healthcare UK Ltd
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Piscataway, NJ 08855-1327, USA
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18-1170-20 AC 05/2006