Cipher DGGE Electrophoresis Systems
Extremely accurate and highly reliable technique for mutation detection
Vertical Comb Options, short wells, overall length = 13.1cm
Cat. #
Cipher DGGE System, 2-place
Cat. # DGGEK-1001
APPLICATIONS
• Detecting single base DNA changes/mutations
• Detecting polymorphisms in double stranded DNA
• Determining DNA fragment melting points
• C.B.S. Scientific offers 4 different sized Cipher DGGE Systems which are
reliable and easy-to-use.
• Superior Temperature stability: Heating element and thermostat maintain
excellent temperature stability (±0.2°) and minimize thermal stratification
during DGGE.
• Buffer Cycler eliminates need of an external peristaltic pump to recycle
buffer
• Can run up to 8 gels at a time.
• This system lends itself well to the use of fluorescence based assays
whose images can be captured on a fluorimeter directly through optional
Borosilicate glass plate sets.
• A mini-peristaltic pump (MPP-100) used in conjunction with the gradient
maker produces accurate and consistent gradient gels
• Single or dual gel cassettes are offered
• Buffer tank dimensions have been reduced to use bench space more
efficiently
• To avoid any incompatibility issues the EPS-300 X (CE) Power Supply
is included because it’s specifications are suited to run all DGGE
applications.
• Simplified casting method using Gel Wrap® gasket for both perpendicular
and vertical gels.
SPECIFICATIONS
Model Cat. #
DGGEK-1001
DGGEK-2001
DGGEK-2401
DGGEK-4801
# of cassettes
1 dual
cassette
2 single
cassettes
2 dual
cassettes
4 dual
cassettes
# of gels
2 gels
2 gels
4 gels
8 gels
gel dimensions
W x L (cm)
15 x 20
15 x 20
15 x 20
15 x 20
footprint
25.4 x 54.6 x 25.4 x 54.6 x
W x L x H (cm)
34.3
34.3
25.4 x 54.6 25.4 x 74.9 x
x 34.3
34.3
# of
Teeth
Thickness
of Teeth
Width of
Teeth
Recommended
Max. Sample
Vol./Well (µl)*
10
10
10
10
10
10
10
10
10
10
10
10
10
10
10
16
20
22
29
30
16
20
22
29
30
16
20
22
29
30
0.75
0.75
0.75
0.75
0.75
1.0
1.0
1.0
1.0
1.0
1.5
1.5
1.5
1.5
1.5
5.2
4.2
4.35
2.9
2.8
5.2
4.2
4.35
2.9
2.8
5.2
4.2
4.35
2.9
2.8
29
23
25
16
16
39
32
32
22
21
58
47
49
33
32
Cat. # DGGEK-2001
Same components as DGGEK-1001 EXCEPT with 2 SINGLE gel cassettes
instead of 1 dual cassette and no buffer displacement tank.
Cipher DGGE System, 4-place
Cat. # DGGEK-2401
• 1 Reinforced buffer tank/lower reservoir with platinum electrodes and
attached anode power lead
• EPS-300 X Power Supply
• 2 dual gel cassettes/upper reservoirs with platinum electrodes
• Safety cover with electrical interlock
• Heater/Stirrer/Buffer Cycler
• 8 sets of spacers (4 sets for vertical DGGE and 4 sets with injection ports for
perpendicular DGGE gel casting)
• 8 combs (4 each 1-well combs and 4 each 16-rectangular well combs)
• Buffer siphon pump
• 4 sets of glass plates
• MPP-100 Mini-peristaltic pump for gradient gel formation
• GM-40 Gradient maker, 20mls per side
• 8 Gel Wrap® gaskets
• White spring clamps
Cipher DGGE System, 8-place
Cat. # DGGEK-4801
• 1 reinforced buffer tank/lower reservoir with platinum electrodes and
attached anode power lead
• EPS-300 X Power Supply
• 4 dual gel cassettes/upper reservoirs with platinum electrodes
• Safety cover with electrical interlock
• Heater/Stirrer/Buffer Cycler
• 16 sets of spacers (8 sets for vertical DGGE and 8 sets with injection ports for
perpendicular DGGE gel casting)
• 16 combs (8 each 1-well combs and 8 each 16-rectangular well combs)
• Buffer siphon pump
• 8 sets of glass plates
• MPP-100 Mini-peristaltic pump for gradient gel formation
• GM-100 Gradient maker, 50mls per side
• 16 Gel Wrap® gaskets
• White spring clamps
REFERENCES
1) S.G. Fischer and L.S. Lerman, (1983) PNAS 80:1579.
2) Guldberg, P., Henriksen, K.F., and Guttler, F. (1993). “Molecular analysis of phenylketonia in
Denmark: 99% of the mutations detected by denaturing gradient gel electrophoresis.” Genomics
17:141-146.
3) Moyret, C., Theillet, C., Puig, P.L., Moles, J-P., Thomas, G. and Hamelin, R. (1994). “Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in
the detection of mutations in exons 5 to 8 of the p53 gene.” Oncogene 9:1739-1743.
w w w. c b s s c i e n t i f i c . c o m
Cat. #
Tooth Depth
(mm)
# of
Teeth
Thickness
of Teeth
Width of
Teeth
Recommended
Max. Sample
Vol./Well (µl)*
19.1
17.7
17.7
17.7
17.7
19.1
19.1
17.7
17.7
17.7
19.1
17.7
17.7
17.7
17.7
16
20
22
29
30
16
20
22
29
30
16
20
22
29
30
0.75
0.75
0.75
0.75
0.75
1.0
1.0
1.0
1.0
1.0
1.5
1.5
1.5
1.5
1.5
5.2
4.2
4.35
2.9
2.8
5.2
4.2
4.35
2.9
2.8
5.2
4.2
4.35
2.9
2.8
55
42
25
29
28
74
55
57
38
37
110
83
85
58
56
VGC-0716
VGC-0720
VGC-0722
VGC-0729
VGC-0730
VGC-1016
VGC-1020
VGC-1022
VGC-1029
VGC-1030
VGC-1516
VGC-1520
VGC-1522
VGC-1529
VGC-1530
*Maximum loading volume is calculated by taking 75% of the total tooth volume.
Gel Casting Options
Option #2: Gel Wrap® Casting System
Option #1: Multi-Caster Kit
C.B.S. Scientific has developed a leak-proof gasket,
Gel Wrap®, that is a simplified method for casting
perpendicular and vertical gels. Gel Wrap® seals
the glass plate sandwich for gel casting quickly and
easily. Each DGGEK System comes with Gel Wrap®
gaskets and all components necessary to cast gels by
this method. See online video instructions: http://www.cbsscientific.com/
instructionvideos.aspx.
Multi-Caster Chamber with
lid, silicone gasket, 6 stainless
steel thumbscrews, backfill port
connecting to central filling
trough, 4 sets notched glass plate
sets, 4 sets of spacers, 4 combs,
polycarbonate separation sheets.
ORDERING INFORMATION
CAT. #
DESCRIPTION
CIPHER DGGE ELECTROPHORESIS SYSTEMS
DGGEK-1001
Cipher DGGE Electrophoresis System, 2-place, 1 dual cassette, Specify 110V or 220V
DGGEK-2001
Cipher DGGE Electrophoresis System, 2-place, 2 single cassettes, Specify 110V or 220V
DGGEK-2401
Cipher DGGE Electrophoresis System, 4-place, 2 dual cassettes, Specify 110V or 220V
DGGEK-4801
Cipher DGGE Electrophoresis System, 8-place, 4 dual cassettes, Specify 110V or 220V
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VGS1520RC-177
VGC-0701-177
VGC-1001-177
VGC-1501-177
VGS-0720R-177
VGS-1020R-177
VGS-1520R-177
VGE-0720-177
VGE-1020-177
VGE-1520-177
NGP-17720NR
NGP-17720DNR
NGP-17720-N-LPF
GCC-404-177K
VGS-0720C-177
VGS-1020C-177
VGS-1520C-177
NGP-17720-N
NGP-17720-DN
NGP-17720-N-LPF
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 0.75mm thickness x 22cm(l)
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 1.0mm thickness x 22cm(l)
Spacer Set for Perpendicular DGGE, standard spacer + injection port spacer, 1.5mm thickness x 22cm(l)
Comb for Perpendicular DGGE, 0.75mm thick x 1 well
Comb for Perpendicular DGGE, 1.0mm thick x 1 well
Comb for Perpendicular DGGE, 1.5mm thick x 1 well
Gel Wrap® Spacer Set, 0.75mm thick x 22cm with rounded bottom corners
Gel Wrap® Spacer Set, 1.0mm thick x 22cm with rounded bottom corners
Gel Wrap® Spacer Set, 1.5mm thick x 22cm with rounded bottom corners
Gel Wrap® Gasket to cast one DGGE gel, 0.75mm thick
Gel Wrap® Gasket to cast one DGGE gel, 1.0mm thick
Gel Wrap® Gasket to cast one DGGE gel, 1.5mm thick
Gel Wrap® Glass plate set, back plate has rounded bottom corners, 17.7cm (w) x 22 cm (h)
Gel Wrap® Glass plate set, Borosilicate back plate with rounded bottom corners, 17.7cm (w) x 22 cm (h)
Gel Wrap® Glass Plate Set, both plates are Borosilicate. Back plate has rounded bottom corners, 17.7cm (w) x 22 cm (h)
Multiple Gel Casting Chamber Kit, 4-place, casts up to 4 each 15cm x 20cm gels
Multiple Gel Casting Spacer Set, 0.75mm thick x 22m(l)
Multiple Gel Casting Spacer Set, 1.0mm thick x 22m(l)
Multiple Gel Casting Spacer Set, 1.5mm thick x 22m(l)
Multiple Gel Casting Chamber Glass Plate Set, 7.7cm(w) x 22cm(h)
Multiple Gel Casting Chamber Glass Plate Set, with Borosilicate back plate, 7.7cm(w) x 22cm(h)
Multiple Gel Casting Chamber Glass Plate Set, both plates are Borosilicate, 7.7cm(w) x 22cm(h)
phone: (858) 755-4959
To l l F r e e : ( 8 0 0 ) 2 4 3 - 4 9 5 9
MUTATION
DETECTION
MUTATION
• Safety cover has electrical interlock for protection and to help maintain
temperature and reduce evaporation
Tooth depth
(mm)
Cipher DGGE System, 2-place
•
FEATURES
DETECTION
• 1 reinforced buffer tank/lower reservoir with platinum electrodes and
attached anode power lead
• EPS-300 X Power Supply
• 1 dual gel cassette/upper reservoir with platinum electrodes
• 1 buffer displacement tank
• Glass safety cover with electrical interlock
• Heater/Stirrer/Buffer Cycler
• 4 sets of spacers (2 sets for vertical DGGE and 2 sets with injection ports for
perpendicular DGGE gel casting)
• 4 combs (2 each 1-well combs and 2 each 16-rectangular well combs)
• Buffer siphon pump
• 2 sets of glass plates
• MPP-100 Mini-peristaltic pump for gradient gel formation
• GM-40 Gradient maker, 20mls per side
• 4 Gel Wrap® gaskets
• White spring clamps
VGC-0716-177
VGC-0720-177
VGC-0722-177
VGC-0729-177
VGC-0730-177
VGC-1016-177
VGC-1020-177
VGC-1022-177
VGC-1029-177
VGC-1030-177
VGC-1516-177
VGC-1520-177
VGC-1522-177
VGC-1529-177
VGC-1530-177
Vertical Comb Options, deeper wells, overall length = 13.1cm